Radiation induced peroxidation of polyunsaturated fatty acids: Recent results on formation of hydroperoxides

Aqueous solutions of linoleic acid were irradiated in air with gamma -rays of Cs-137. High pressure liquid chromatography (HPLC) was been used to sepa rate and measure the production of hydroperoxides. The results obtained aft er reverse phase chromatography, associated with a microperoxydase for hydr operoxide detection, indicate the presence of two different hydroperoxides. One type of hydroperoxide was the major product obtained when the initial linoleic concentrations were below the critical micellar concentration (2 m M), and the second type was produced when the concentrations were above 2 m M. A further separation carried out on the second hydroperoxide by direct p hase HPLC showed that it contains three compounds, mainly HPODE 9 and 13.