Adenoviral SERCA1a gene transfer to adult rat ventricular myocytes inducesphysiological changes in calcium handling

Objective: We examined the functional consequences of expressing adult rabb it fast skeletal sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA1a) in isola ted adult rat ventricular myocytes. Methods: Myocytes were infected with a recombinant adenovirus harboring SERCA1a. Then 2 days after myocyte infecti on, protein expression was estimated using Western blot and SDS-PAGE analys is. We also measured the ATP-dependent oxalate-facilitated Ca2+ uptake of m yocyte homogenates and monitored Ca2+ transient in myocytes loaded with the Ca2+ dye, indo-1. Results: SERCA1a gene expression resulted in a 36% incre ase in the total SERCA protein level in infected myocytes compared to contr ols (P<0.01), white SERCA2 and phospholamban levels did not change. This in crease was associated with a 42% rise in SR Ca2+ uptake (P<0.01), while tau (the time constant of Ca2+ transient decay), and the time to peak fell by 32% (P<0.01) and 38% (P<0.001), respectively. Increasing the frequency of s timulation from 0.2 to 2 Hz decreased tau in both cell types (P<0.01). Howe ver, the decrease was much smaller in infected (P<0.01) than in uninfected cells (P<0.001). Isoproterenol (1 <mu>M) further decreased tau in infected myocytes by 23% (P<0.05). In these cells, the diastolic [Ca2+](i) decreased by 50% (P<0.05) while the systolic [Ca2+](i) increased by 19% (P<0.05). No difference was found in the speed of SR Ca2+ reloading after caffeine wash out between the two cell types. Conclusion: Adenovirus-mediated SERCA1a gen e transfer to adult rat ventricular myocytes enhances SR Ca2+ handling to a degree similar to that observed following physiological stimulation. (C) 2 001 Elsevier Science B.V. All rights reserved.