Effect of cisplatin on mitochondrial protein, glutathione, and succinate dehydrogenase in Dalton lymphoma-bearing mice

Cisplatin treatment of tumor-bearing mice resulted a significant decrease o f protein in the tissues studied (liver, kidney, and Dalton lymphoma) and a lso in their mitochondrial fractions. As compared to respective tissues, th e protein decrease was noted to be more conspicuous in their mitochondrial fractions. Similarly, mitochondrial glutathione also decreased significantl y in the tissues. However, succinate dehydrogenase activity was selectively decreased in the kidney and Dalton lymphoma cells, whereas in liver it rem ained almost unchanged. An increase in serum urea concentration and kidney mitochondrial lipid peroxidation was also observed after cisplatin treatmen t. It is suggested that the cisplatin-induced biochemical changes in mitoch ondria involving mitochondrial protein, glutathione, and succinate dehydrog enase could be the important potent cellular sites contributing to toxicity /cytotoxicity after cisplatin treatment.