Bcl-2 induces cyclin D-1 promoter activity in human breast epithelial cells independent of cell anchorage

Cyclin D-1 expression is co-regulated by growth factor and cell adhesion si gnaling. Cell adhesion to the extracellular matrix activates focal adhesion kinase (FAK), which is essential for cyclin D-1 expression. Upon the loss of cell adhesion, cyclin D-1 expression is downregulated, followed by apopt osis in normal epithelial cells. Since bcl-2 prevents apoptosis induced by the loss of cell adhesion, we hypothesized that bcl-2 induces survival sign aling complementary to cell adhesion-mediated gene regulation. In the prese nt study, we investigated the role of bcl-2 on FAK activity and cyclin D-1 expression. We found that bcl-2 overexpression induces cyclin D-1 expressio n in human breast epithelial cell line MCF10A independent of cell anchorage . Increased cyclin D-1 expression in stable bcl-2 transfectants is not rela ted to bcl-2-increased G(1) duration, but results from cyclin D-1 promoter activation, Transient transfection studies confirmed anchorage-independent bcl-2 induction of cyclin D-1 promoter activity in human breast epithelial cell lines (MCF10A, BT549, and MCF-7), We provide evidence that bcl-2 induc tion of cyclin D-1 expression involves constitutive activation of focal adh esion kinase, regardless of cell adhesion. The present study suggests a pot ential oncogenic activity for bcl-2 through cyclin D-1 induction, and provi des an insight into the distinct proliferation-independent pathway leading to increased cyclin D-1 expression in breast cancer.