M. Sandbacka et B. Isomaa, Glutathione S-transferase and UDP-glucuronyltransferase activity in primary cultures of rainbow trout gill epithelial cells, COMP BIOC C, 127(3), 2000, pp. 307-315
Citations number
42
Categorie Soggetti
Pharmacology & Toxicology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
The objective of this study was to characterize rainbow trout (Oncorhynchus
mykiss) fill epithelial cells in primary culture by evaluating their abili
ty to maintain glutathione and glucuronide conjugating enzymes. The activit
y and inducibility of the phase II enzymes was investigated as a function o
f culture time. Glutathione S-transferase (GST) and UDP-glucuronyltransfera
se (UDPGT) enzyme activities were measured in freshly isolated cells and in
cells cultured for 7 and 12 days. GST activity, determined with 1-chloro-2
,4-dinitrobenzene, decreased gradually to 72% after 7 days and to 38% after
12 days in culture compared with freshly isolated cells. There was no sign
ificant difference between UDPGT activities in freshly isolated cells compa
red with cells cultured up to 12 days although a transient decrease in acti
vity was observed at day 7. In vitro induction of the enzymes was studied u
sing beta -naphtoflavone (BNF) and 3-methylcholanthrene (3-MC) as inducers.
GST activity increased 2-fold after exposure to BNF and 1.5-fold after 3-M
C exposure for 48 h in 7 days old cultures. No induction was observed in 12
days old cultures. UDPGT activity was not induced either at day 7 or 12. (
C) 2000 Elsevier Science Inc. All rights reserved.