Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin

Authors
Fukuda, N Tanaka, H Shoyama, Y
Citation
N. Fukuda et al., Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin, CYTOTECHNOL, 34(3), 2000, pp. 197-204
Citations number
33
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
CYTOTECHNOLOGY
ISSN journal
09209069 → ACNP
Volume
34
Issue
3
Year of publication
2000
Pages
197 - 204
Database
ISI
SICI code
0920-9069(200011)34:3<197:FOMAAA>2.0.ZU;2-5
Abstract
The ratio of hapten and bovine serum albumin in an antigen conjugate was de termined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenosid e Rg(1) was produced by fusing sprenocytes immunized with a ginsenoside Rg( 1)-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell lin e, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re . The full measuring range of the assay extends from 0.3 mug ml(-1) to 10 m ug ml(-1) of ginsenoside Rg(1).