The aim of this study was to apply a cortisol metabolite determination in t
he faeces of cats and dogs for monitoring disturbances. In this experiment
faeces from every spontaneous defecation of 10 cats and 10 dogs (5 of each
sex) were collected starting from one day before until two days after the y
early vaccination. Concentrations of 11,17-dioxoandrostanes (cat) and corti
sol equivalents (dog) were determined by enzyme immunoassay (EIA). Faecal c
ortisol metabolites increased and reached peak concentrations (median: 412
% in cats and 417 % in dogs, respectively above baseline values) in one of
the next two samples following the vaccination. This indicated an activatio
n of the adrenocortex, the degree to which the different parts (physical an
d psychological components) of the whole vaccination procedure contributed
to it was not evaluated. From this experiment we conclude that measuring co
rtisol metabolites in the faeces is a non-invasive method to monitor stress
ful conditions in cats and dogs and thus is a valuable tool for evaluating
animal welfare.