beta(3)-Adrenergic receptor gene polymorphism and type 2 diabetes in a Caucasian population

Aim: The beta (3)-adrenergic receptor (beta (3)-AR) is suspected to play a key role in the regulation of energy balance by increasing lipolysis and th ermogenesis. A mutation in the beta (3)-AR gene (Trp64Arg) has been associa ted with the capacity of weight gain and with early onset of noninsulin dep endent diabetes mellitus (type 2 diabetes). In this study we investigated t he prevalence of the two beta (3)-AR alleles in a Caucasian population and studied the association between the beta (3)-AR genotype and metabolic diso rders (obesity and type 2 diabetes). Methods: Genomic DNA extracted from peripheral blood leucocytes of 200 Cauc asian subjects (137 subjects with and 63 subjects without type 2 diabetes). The MvaI polymorphism of beta (3)-AR, which detects the Trp64Arg mutation, was determined by polymerase chain reaction (PCR). We studied the correlat ion between the Trp64Arg mutation and the body mass index (b.m.i. kg/m(2)). Results: There was no significant difference between the patients with type 2 diabetes and control subjects in the frequency of the Arg64 allele (5.5% and 4.8%, respectively). Within the group of type 2 diabetes patients were 14 subjects with the Trp64Arg mutation (b.m.i., mean +/- s.d.: 31 +/- 8.5 kg/m(2)) and 123 without the mutation (b.m.i. 29 +/- 4.8). There was no ass ociation between the beta (3)-AR gene polymorphism and sex, obesity, blood pressure, glycohaemoglobin concentration, proteinuria. Conclusion: Our results suggest that the Trp64Arg mutation is not a major d eterminant of metabolic disorders (type 2 diabetes, obesity) and chronic co mplications of type 2 diabetes in a Dutch population.