Distribution of the hallucinogens N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine in rat brain following intraperitoneal injection: application of a new solid-phase extraction LC-APcI-MS-MS-isotope dilution method

A method for the solid-phase extraction (SPE) and liquid chromatographic-at mospheric pressure chemical ionization-mass spectrometric-mass spectrometri c-isotope dilution (LC-APcI-MS-MS-ID) analysis of the indole hallucinogens N,N-dimethyltryptamine (DMT) and 5-methoxy DMT (or O-methyl bufotenin, OMB) from rat brain tissue is reported. Rats were administered DMT or OMB by th e intraperitoneal route at a dose of 5 mg/kg and sacrificed 15 min post tre atment. Brains were dissected into discrete areas and analyzed by the metho ds described as a demonstration of the procedure's applicability. The synth esis and use of two new deuterated internal standards for these purposes ar e also reported. (C) 2001 Elsevier Science B.V. All rights reserved.