High-performance liquid chromatographic assays for a second-generation novel oral iron chelator (APCP363) and their application to pharmacokinetic studies in rats

Sensitive and specific HPLC assays for APCP363 in biological matrices (rat plasma, urine and feces) were developed. The recovery of APCP363 ranged fro m 81.2 to 99.9% in plasma, from 82.1 to 92.8% in urine, and from 65 to 68% in feces. Standard deviations were below 10% for all analyses. The limits o f quantitation were 0.1, 10 and 30 mug/ml in plasma, urine and feces, respe ctively. The HPLC assays, which are the first reports for APCP363 analysis in biological matrices, have been successfully applied to preliminary pharm acokinetic studies in rats. The stool assay is the first non-radiolabeled m ethod for hydroxypyridinones in feces. (C) 2001 Elsevier Science B.V. All r ights reserved.