Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR test and manual microwell plate-based AMPLICOR HBV MONITOR test

Authors
Marin, IJ Poljak, M Seme, K Meglic-Volikar, J Maticic, M Lesnicar, G Brinovec, V
Citation
Ij. Marin et al., Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR test and manual microwell plate-based AMPLICOR HBV MONITOR test, J CLIN MICR, 39(2), 2001, pp. 758-761
Citations number
22
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
2
Year of publication
2001
Pages
758 - 761
Database
ISI
SICI code
0095-1137(200102)39:2<758:CEOSCA>2.0.ZU;2-F
Abstract
Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B viru s (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPL ICOR-HBV) on 208 serum samples revealed no significant difference in the se nsitivities of the two assays. Twenty samples tested HBV DNA negative and 1 83 samples tested HBV DNA positive by both assays. Three samples tested pos itive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV onl y. HBV DNA concentrations determined by the two assays were significantly r elated (n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV coul d replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the require d performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.