Quantification of human cytomegalovirus DNA by real-time PCR

Authors
Gault, E Michel, Y Dehee, A Belabani, C Nicolas, JC Garbarg-Chenon, A
Citation
E. Gault et al., Quantification of human cytomegalovirus DNA by real-time PCR, J CLIN MICR, 39(2), 2001, pp. 772-775
Citations number
18
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
2
Year of publication
2001
Pages
772 - 775
Database
ISI
SICI code
0095-1137(200102)39:2<772:QOHCDB>2.0.ZU;2-M
Abstract
A quantitative real-time PCR assay was developed to measure human cytomegal ovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular ge ne (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay (P < 0.0001).