Immunofluorescence method for detection of Ebola virus immunoglobulin G, using HeLa cells which express recombinant nucleoprotein

Authors
Saijo, M Niikura, M Morikawa, S Kurane, I
Citation
M. Saijo et al., Immunofluorescence method for detection of Ebola virus immunoglobulin G, using HeLa cells which express recombinant nucleoprotein, J CLIN MICR, 39(2), 2001, pp. 776-778
Citations number
20
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
2
Year of publication
2001
Pages
776 - 778
Database
ISI
SICI code
0095-1137(200102)39:2<776:IMFDOE>2.0.ZU;2-B
Abstract
A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleopro tein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expre ssed EBO NP, and this was used as an immunofluorescent (IF) antigen to dete ct EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulnes s in the diagnosis of EBO infections and seroepidemiological studies.