Detection of Legionella pneumophila using a real-time PCR hybridization assay

Authors
Ballard, AL Fry, NK Chan, L Surman, SB Lee, JV Harrison, TG Towner, KJ
Citation
Al. Ballard et al., Detection of Legionella pneumophila using a real-time PCR hybridization assay, J CLIN MICR, 38(11), 2000, pp. 4215-4218
Citations number
17
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
11
Year of publication
2000
Pages
4215 - 4218
Database
ISI
SICI code
0095-1137(200011)38:11<4215:DOLPUA>2.0.ZU;2-P
Abstract
A real-time PCR hybridization assay for Legionella pneumophila is described ; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water s ample. The assay, including DNA extraction and confirmation of product iden tity, is completed within 90 min of receipt of a sample.