I. Rey-gomez-serranillos et al., Desorption of amphotericin B from mixed monolayers with cholesterol at theair/water interface, J COLL I SC, 234(2), 2001, pp. 351-355
The desorption of amphotericin B (AmB) from mixed monolayers with cholester
ol was studied by monitoring temporal changes in the relative area (A(t)/A(
o)) as a function of time at constant surface pressures, corresponding to t
hree distinct regions of the AmB isotherm, The loss of AmB molecules from t
he interface was found to obey the Ter Minassian Saraga model [J, Colloid I
nterface Sci. ii, 398 (1956)], In the low surface pressure region the overa
ll process of desorption is controlled by a one-step dissolution (at small
times) followed by diffusion. At high pressures, however, the process of di
ssolution is more complex and consists of two steps that differ in rate. Th
e presence of cholesterol molecules in the monolayer prevents the desorptio
n of AmB molecules as evidenced by the constant desorption values. The anal
ysis of the kinetics parameters confirms our previous findings, based on th
e analysis of excess functions, that the highest interactions, due to the s
table complex formation, appear for the mixture of X-AmB = 0.7 (C) 2001 Aca
demic Press.