In this study, we evaluated the effectiveness of prostaglandin (PG) E-1 in
inhibiting wound contraction, both alone and in combination with collagen m
atrix, using a in vivo full thickness skin defect model. To clarify the mec
hanisms involved in this inhibition we also used a fibroblast-populated col
lagen gel contraction in vitro model. We demonstrated that collagen matrix
alone significantly inhibited wound contraction PG E-1 alone did not. Inter
estingly, their combination was much more effective than either collagen ma
trix or PG E-1 alone, a finding which was also supported by histopathologic
al examination. Wounds treated with collagen matrix, but not control wounds
, showed horizontal rearrangement of collagen fibers in the dermal part as
well as evidence of active fibroblast proliferation which was not observed
in scar regions surrounded by normal dermis. With the fibroblast-populated
collagen gel contraction in vitro model, we found that PG E-1 significantly
inhibited contraction at a high dose. It was concluded that collagen matri
x combined with PG E-1 is effective for preventing contracture producing so
called neodermis than collagen matrix alone, which remains one of the most
challenging problems in treating full thickness type wounds. (C) 2001 Else
vier Science Ireland Ltd. All rights reserved.