Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.)

Microsatellite or simple sequence repeat (SSR) markers have been successful ly used for genomic mapping, DNA fingerprinting, and marker-assisted select ion in many plant species, Here we report the first successful assignment o f 15 SSR markers to the Phaseolus vulgaris molecular linkage map, A total o f 37 SSR primer pairs were developed and tested for amplification and produ ct-length polymorphism with BAT93 and Jalo EEP558, the parental lines of an F-7 recombinant inbred (RI) population previously used for the constructio n of a common bean molecular linkage map, Sixteen of the SSRs polymorphic t o the parental lines were analyzed for segregation and 15 of them were assi gned to seven different linkage groups, indicating a widespread distributio n throughout the bean genome, Map positions for genes coding for DNAJ-like protein, pathogenesis-related protein 3, plastid-located glutamine syntheta se, endochitinase, sn-glycerol-3 phosphate acyltransferase, NADP-dependent malic enzyme, and protein kinase were determined for the first time, Additi on of three SSR loci to linkage group B4 brought two separated smaller link age groups together to form a larger linkage group, Analysis of allele segr egation in the F-7 RI population revealed that all 16 SSRs segregated in th e expected 1:1 ratio, These SSR markers were stable and easy to assay by po lymerase chain reaction (PCR), They should be useful markers for genetic ma pping, genotype identification, and marker-assisted selection of common bea ns.