L. Muskhelishvili et al., In situ hybridization and immunohistochemical analysis of cytochrome P4501B1 expression in human normal tissues, J HIST CYTO, 49(2), 2001, pp. 229-236
Cytochrome P450 1B1 (CYP1B1) is a recently cloned dioxin-inducible form of
the cytochrome P450 supergene family of xenobiotic-metabolizing enzymes. CY
P1B1 is constitutively expressed mainly in extrahepatic tissues and is indu
cible by aryl hydrocarbon receptor ligands. Human CYP1B1 is involved in act
ivation of chemically diverse human procarcinogens, including polycyclic ar
omatic hydrocarbons and some aromatic amines, as well as the endogenous hor
mone 17 beta -estradiol. The metabolism of 17 beta -estradiol by CYP1B1 for
ms 4-hydroxyestradiol, a product believed to be important in estrogen-induc
ed carcinogenesis. Although the distribution of CYP1B1 mRNA and protein in
a number of human normal tissues has been well documented, neither the cell
s expressing CYP1B1 in individual tissue nor the intracellular localization
of the enzyme has been thoroughly characterized. In this study, using nonr
adioactive in situ hybridization and immunohistochemistry, we examined the
cellular localization of CYP1B1 mRNA and protein in a range of human normal
tissues. CYP1B1 mRNA and protein were expressed in most samples of parench
ymal and stromal tissue from brain, kidney, prostate, breast, cervix, uteru
s, ovary, and lymph nodes. In most tissues, CYP1B1 immunostaining was nucle
ar. However, in tubule cells of kidney and secretory cells of mammary gland
, immunoreactivity for CYP1B1 protein was found in both nucleus and cytopla
sm. This study demonstrates for the first time the nuclear localization of
CYP1B1 protein. Moreover, the constitutive expression and wide distribution
of CYP1B1 mRNA and protein in many human normal tissues suggest functional
roles for CYP1B1 in the bioactivation of xenobiotic procarcinogens and end
ogenous substrates such as estrogens.