Jw. Choi et al., LB FILM CONTAINING ACETYLCHOLINESTERASE FOR FIBEROPTIC ORGANOPHOSPHORUS SENSOR, Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals, 294, 1997, pp. 451-454
LB film containing Acetylcholinesterase(AChE) was fabricated for the d
evelopment of the fiber-optic biosensor to detect organophosphorus com
pounds in the contaminated water. The AChE-immobilized LB film was for
med by adsorbing the enzyme molecules onto viologen monolayer using th
e electrostatic force. The optimal amount of the dissolved enzyme for
the LB film formation were determined based on the enzyme adsorption i
sotherm and relative adsorption amount. The surface characteristics of
AChE-immobilized LB film was analyzed by the atomic force microscope(
AFM). The surface topography of the AChE-immobilized viologen LB films
were obtained for the various conditions of the surface pressure. The
response time to steady signal and the detection range of the sensor
were 5 min and 0 similar to 2 ppm, respectively.