LB FILM CONTAINING ACETYLCHOLINESTERASE FOR FIBEROPTIC ORGANOPHOSPHORUS SENSOR

LB film containing Acetylcholinesterase(AChE) was fabricated for the d evelopment of the fiber-optic biosensor to detect organophosphorus com pounds in the contaminated water. The AChE-immobilized LB film was for med by adsorbing the enzyme molecules onto viologen monolayer using th e electrostatic force. The optimal amount of the dissolved enzyme for the LB film formation were determined based on the enzyme adsorption i sotherm and relative adsorption amount. The surface characteristics of AChE-immobilized LB film was analyzed by the atomic force microscope( AFM). The surface topography of the AChE-immobilized viologen LB films were obtained for the various conditions of the surface pressure. The response time to steady signal and the detection range of the sensor were 5 min and 0 similar to 2 ppm, respectively.