Pharmacokinetics and bioavailability of alpha-, gamma- and delta-tocotrienols under different food status

We have investigated the pharmacokinetics and bioavailability of alpha-, ga mma- and delta -tocotrienols under fed and fasted conditions in eight healt hy volunteers. The volunteers were administered a single oral dose of mixed tocotrienols ( 300 mg) under fed or fasted conditions. The bioavailability of tocotrienols under the two conditions was compared using the parameters peak plasma con centration (C-max), time to reach peak plasma concentration (T-max) and tot al area under the plasma concentration-time curve (AUC(0-infinity)). A statistically significant difference was observed between the fed and fas ted logarithmic transformed values of C-max (P < 0.01) and AUC(0-<infinity> ) (P < 0.01) for all three tocotrienols. In addition, the 90% confidence in tervals for the ratio of the logarithmic transformed AUC(0-<infinity>) valu es of alpha-, gamma- and delta -tocotrienols under the fed state over those of the fasted state were found to lie between 2.24-3.40, 2.05-4.09 and 1.5 9-3.81, respectively, while those of the C-max were between 2.28-4.39, 2.31 -5.87 and 1.52-4.05, respectively. However, no statistically significant di fference was observed between the fed and fasted T-max values of the three homologues. The mean apparent elimination half-life (t(1/2)) of alpha-, gam ma- and delta -tocotrienols was estimated to be 4.4, 4.3 and 2.3 h, respect ively, being between 4.5- to 8.7-fold shorter than that reported for alpha -tocopherol. No statistically significant difference was observed between t he fed and fasted t(1/2) values. The mean apparent volume of distribution ( Vd/f) values under the fed state were significantly smaller than those of t he fasted state, which could be attributed to increased absorption of the t ocotrienols in the fed state.