Isolation and characterisation of DNA from whale bone

Citation
Sj. Tebbutt et al., Isolation and characterisation of DNA from whale bone, J RS NZ, 30(4), 2000, pp. 365-371
Citations number
11
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF THE ROYAL SOCIETY OF NEW ZEALAND
ISSN journal
03036758 → ACNP
Volume
30
Issue
4
Year of publication
2000
Pages
365 - 371
Database
ISI
SICI code
0303-6758(200012)30:4<365:IACODF>2.0.ZU;2-K
Abstract
DNA was extracted from a sperm whale rib bone, and an ear bone from an unid entified large baleen whale. Prior to extraction the two bones had been sub jected to various environmental conditions resulting in limited decompositi on of the bone material. Specific mitochondrial DNA sequences were amplifie d from the extracts using the technique of polymerase chain reaction. Subse quent sequencing of these products confirmed that the rib bone was indeed f rom a sperm whale, and that the ear bone was from a blue whale. The results show that genetic information can be obtained from whale bones that have b een exposed to the elements.