Molecular characterization in the VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) isolated in Japan and Kenya

Authors
Kudo, S Zhou, Y Cao, XR Yamanishi, S Nakata, S Ushijima, H
Citation
S. Kudo et al., Molecular characterization in the VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) isolated in Japan and Kenya, MICROB IMMU, 45(2), 2001, pp. 167-171
Citations number
25
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
03855600 → ACNP
Volume
45
Issue
2
Year of publication
2001
Pages
167 - 171
Database
ISI
SICI code
0385-5600(2001)45:2<167:MCITVV>2.0.ZU;2-8
Abstract
The VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) were analyze d to investigate intraserotypic variations. The techniques used included re verse transcription polymerase chain reaction with subtype specific primers , restriction fragment length polymorphism analysis and sequence analysis, Twelve isolates (nine from Japan and three from Kenya) and two standard str ains (Hochi, Odelia) were G4A P[8] Wa group NSP4, A standard strain (ST3) w as G4A P[6] Wa group NSP4 and a strain (VA70) was GIB P[8] Wa group NSP4. T hese results show G4 rotaviruses can be divided into three combinations at the moment.