Genomic map of Clostridium perfringens strain 13

A physical and genetic map of Clostridium perfringens strain 13 was constru cted. C, perfringens strain 13 was found to have a 3.1-Mb chromosome and a large 50-kb plasmid, indicating that strain 13 has a relatively small genom e among C, perfringens strains. A total of 313 genetic markers were mapped on the chromosome of strain 13, Compared with the physical and genetic map of C, perfringens CPN50, strain 13 had a quite similar genome organization, but with a large deletion (similar to 400 kb) in a particular segment of t he chromosome. Among several toxin genes, a beta2 toxin gene that is a nove l virulence gene in C. perfringens was found to be located on the 50-kb pla smid.