HPLC-DAD determination of Metformin in human plasma using derivatization with p-nitrobenzoyl chloride in a biphasic system

Authors
Tache, F David, V Farca, A Medvedovici, A
Citation
F. Tache et al., HPLC-DAD determination of Metformin in human plasma using derivatization with p-nitrobenzoyl chloride in a biphasic system, MICROCHEM J, 68(1), 2001, pp. 13-19
Citations number
8
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
MICROCHEMICAL JOURNAL
ISSN journal
0026265X → ACNP
Volume
68
Issue
1
Year of publication
2001
Pages
13 - 19
Database
ISI
SICI code
0026-265X(200101)68:1<13:HDOMIH>2.0.ZU;2-Z
Abstract
An optimized method for determination of Metformin at ppb level in human pl asma is described. Derivatization of Metformin with p-nitrobenzoyl chloride was carried out in a biphasic system. The derivative extracted in the orga nic layer (dichloromethane) was concentrated and analyzed by HPLC-DAD, usin g an isocratic elution (water/methanol mixture 65/35), monitored at 280 nm. Good selectivity against co-extracted matrix components was observed. A de tection limit of 10 ppb Metformin in human plasma was reached. (C) 2001 Els evier Science B.V. All rights reserved.