Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis

Authors
Stahl, F Bowers, R Mooney, D Myers, R Stahl, M Thomason, L
Citation
F. Stahl et al., Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis, MOL G GENET, 264(5), 2001, pp. 716-723
Citations number
22
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR AND GENERAL GENETICS
ISSN journal
00268925 → ACNP
Volume
264
Issue
5
Year of publication
2001
Pages
716 - 723
Database
ISI
SICI code
0026-8925(200101)264:5<716:GAROPL>2.0.ZU;2-D
Abstract
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lyric cycle, are not blocked for growth. The blocked-growth phenotyp e of lambda ea47(+) in the presence of AddAB is expressed only when phage D NA replication is permitted.