In pelagic egg spawners, the production of large numbers of sinking eggs, u
nable to develop into embryos, represents one of the major limiting factors
in controlled reproduction. The aim of this study is to elucidate the mole
cular differences between floating and nonfloating eggs at cytoplasmic and
nuclear level. Comparison of analyses between floating and nonfloating sea
bream Sparus aurata eggs evidenced differences in vitelline envelope protei
n components, such differences being probably related with the hydration pr
ocess but not with fertilization as supported by the assessment of DNA that
doubled after in vitro insemination. These data clearly indicated that the
absence of embryo development in nonfloating eggs is not due to lack of fe
rtilization. The cytoplasmic composition was also different, the number of
protein components being higher in floating eggs, and these extra component
s may generate the appropriate osmotic pressure at the base of the hydratio
n process. Some lysosomal enzymes, such as cathepsin D and L both involved
in yolk proteolysis, in virgin nonfloating eggs were significantly higher w
ith respect to floating ones; the levels of these two enzymes significantly
increased in the latter after fertilization. On the contrary, in nonfloati
ng eggs cathepsin L significantly decreased after fertilization. These chan
ges may be related with a series of metabolic processes vital for the produ
ction of viable offspring. The capacity of egg transcription and the protei
n synthesis in these two types of eggs, indicated by the RNA/DNA and RNA/pr
otein ratios, evidenced that the status of cell transcription rate and prot
ein synthesis capacity is significantly higher in floating eggs. This, in t
urn, suggested that the lack of embryo development may be due to low levels
of proteins involved in cell cycle regulation. (C) 2001 Wiley-Liss, Inc.