An efficient synthetic strategy for the preparation of nucleic acid-encoded peptide and protein libraries for in vitro evolution protocols

We describe an improved synthetic strategy for the preparation of nucleic a cid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the prep aration of fusion libraries and should prove most useful for in vitro prote in evolution procedures which involve repetitive cycles of fusion library p reparation and selection.