K. Adamsky et al., Glial tumor cell adhesion is mediated by binding of the FNIII domain of receptor protein tyrosine phosphatase beta (RPTP beta) to tenascin C, ONCOGENE, 20(5), 2001, pp. 609-618
The extracellular domain of receptor protein tyrosine phosphatase beta (RPT
P beta) is composed of several domains which mediate its interactions with
distinct ligands present on the surface of either neurons or glial cells. H
ere, we demonstrate that the fibronectin type III domain (FNIII) of RPTP be
ta binds to glial tumor-derived cell lines and primary astrocytes, We used
affinity purification to isolate several proteins that specifically bind to
the FNIII domain of RPTP beta. One of these, a 240 kDa protein that was pu
rified from U118MG glioblastoma cell, was identified as tenascin C based on
the amino acid sequence of several tryptic peptides. The interaction of RP
TP beta with tenascin C was found to mediate cell adhesion. Adhesion and sp
reading of SF763T astrocytoma cells expressing RPTP beta on tenascin C,was
specifically abolished by the addition of a soluble fragment containing the
FNIII domain of the receptor, RPTP beta -dependent cell adhesion was media
ted by binding to the alternatively spliced FNIII repeats A1,2,4 (TnfnA1,2,
4) of tenascin C, Furthermore, COS cells expressing RPTP beta adhere to Tnf
nA1,2,4, while the parental cells did not. These results demonstrate that t
he FNIII domain of RPTP beta binds to tenascin C and suggest that RPTP beta
present on glial tumor cells is a primary adhesion receptor system to the
extracellular matrix.