Drinks like a fish: zebra fish (Danio rerio) as a behavior genetic model to study alcohol effects

Zebra fish may be an ideal vertebrate model system for numerous human disea ses with which the genetics and biological mechanisms of the disease may be studied. Zebra fish has been successfully used in developmental genetics, and recently, neurobiologists have also started to study this species. A po tentially interesting target disease amenable for analysis with zebra fish is drug addiction, e.g. alcoholism. Although genetic tools to manipulate th e genome of zebra fish are available, appropriate phenotypical testing meth ods are often lacking. In this paper, we describe basic behavioral tests to investigate the acute effects of alcohol on zebra fish. These behavioral p aradigms will be useful for the genetic and biological analysis of acute an d chronic drug effects as well as addiction. In addition to presenting find ings for the acute effects of alcohol, we briefly describe our strategy for generating and screening mutants. We hope that our pilot work will facilit ate the future development of behavioral tests and the use of zebra fish in the genetic analysis of the biological effects of drugs of abuse. (C) 2001 Elsevier Science Inc. All rights reserved.