P. Luo et al., A study on floral biology of seedlings in vitro in Orychophragmus violaceus: Induction of flowers in seedlings of O-violaceus cultured in vitro, PL CELL TIS, 63(1), 2000, pp. 73-75
Seedlings of O. violaceus were cultured on MS media and treated at low temp
erature. Cold treatment at 5-7 degreesC for more than 7 days was needed for
flower induction of seedlings in vitro originated from germinated seeds. W
hen cultured on MS media supplemented with 2.5 mg l(-1) zeatin and 2 mg l(-
1) gibberellin (GA(3)), seedlings in vitro did initiate flowers without col
d treatment. When MS media was used with a reduced amount of NH4NO3, flower
induction of seedlings in vitro could be accelerated.