Fertile and diploid nuclear transplants derived from embryonic cells of a small laboratory fish, medaka (Oryzias latipes)

Fertile and diploid nuclear transplants were successfully generated by usin g embryonic cells as donors in a small laboratory fish, medaka (Oryzias lat ipes), Embryonic cell nuclei from transgenic fish carrying the green fluore scent protein (GFP) gene were transplanted into unfertilized eggs enucleate d by x-ray irradiation. In this study, I out of 588 eggs transplanted in th e first experiment and 5 out of 298 eggs transplanted in the second experim ent reached the adult stage, Ail of these nuclear transplants were fertile and diploid, and the natural and GFP markers of the donor nuclei were trans mitted to the F-1 and F-2 offspring in a Mendelian fashion. This systematic study proves the feasibility of generating nuclear transplants by using em bryonic cells from fish as donors, and it is supported by convincing eviden ce.