In vivo model mimicking natural history of dog prostate cancer using DPC-I, a new canine prostate carcinoma cell line

Citation
M. Anidjar et al., In vivo model mimicking natural history of dog prostate cancer using DPC-I, a new canine prostate carcinoma cell line, PROSTATE, 46(1), 2001, pp. 2-10
Citations number
16
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
PROSTATE
ISSN journal
02704137 → ACNP
Volume
46
Issue
1
Year of publication
2001
Pages
2 - 10
Database
ISI
SICI code
0270-4137(20010101)46:1<2:IVMMNH>2.0.ZU;2-E
Abstract
BACKGROUND. Dog prostate cancer is usually considered to be highly relevant to human prostate cancer. We report the isolation of a new canine prostate cancer epithelial cell line designated DPC-1. METHODS. Primary cultures were established from a canine poorly differentia ted prostatic adenocarcinoma. Population doubling time was determined by co unting nuclei after cell lysis. Tumorigenicity was assessed in nude mice an d in one adult immunodeficient dog. Immunoscintigraphy was performed in bot h models using a monoclonal antibody (mAb) raised against the [44-62] seque nce of human PSMA. RESULTS. DPC-1 cells have a rapid growth in vitro (doubling time, 27 hr) wh ich is not stimulated by androgens. In addition, DPC-1 displays immunoreact ivity to human PSA and PSMA. DPC-1 was found to be highly tumorigenic not o nly in nude mice but also for the first time after orthotopic seeding in an immunodeficient dog. This allograft mimicked, in a compressed form, the ag gressive biological behavior of spontaneous dog prostate adenocarcinoma. Im munoscintigraphy using a (131)Iodine-labeled PSMA mAb clearly visualized in duced tumors in nude mice and in the dog allograft. CONCLUSIONS. This study suggests that DPC-1 may constitute a powerful model for assessing new diagnostic and/or therapeutic tools in the management of prostate cancer. Prostate 46:2-10, 2001. (C) 2001 Wiley-Liss, Inc.