M. Anidjar et al., In vivo model mimicking natural history of dog prostate cancer using DPC-I, a new canine prostate carcinoma cell line, PROSTATE, 46(1), 2001, pp. 2-10
BACKGROUND. Dog prostate cancer is usually considered to be highly relevant
to human prostate cancer. We report the isolation of a new canine prostate
cancer epithelial cell line designated DPC-1.
METHODS. Primary cultures were established from a canine poorly differentia
ted prostatic adenocarcinoma. Population doubling time was determined by co
unting nuclei after cell lysis. Tumorigenicity was assessed in nude mice an
d in one adult immunodeficient dog. Immunoscintigraphy was performed in bot
h models using a monoclonal antibody (mAb) raised against the [44-62] seque
nce of human PSMA.
RESULTS. DPC-1 cells have a rapid growth in vitro (doubling time, 27 hr) wh
ich is not stimulated by androgens. In addition, DPC-1 displays immunoreact
ivity to human PSA and PSMA. DPC-1 was found to be highly tumorigenic not o
nly in nude mice but also for the first time after orthotopic seeding in an
immunodeficient dog. This allograft mimicked, in a compressed form, the ag
gressive biological behavior of spontaneous dog prostate adenocarcinoma. Im
munoscintigraphy using a (131)Iodine-labeled PSMA mAb clearly visualized in
duced tumors in nude mice and in the dog allograft.
CONCLUSIONS. This study suggests that DPC-1 may constitute a powerful model
for assessing new diagnostic and/or therapeutic tools in the management of
prostate cancer. Prostate 46:2-10, 2001. (C) 2001 Wiley-Liss, Inc.