Nitrate potentially has a major impact on the quality of groundwater for po
table supply. Conventional treatment methods to control nitrate problems in
abstracted groundwater are expensive, in terms of unit costs and environme
ntal impact. utilizing the natural denitrifying capacity of aquifers offers
an alternative approach to nitrate management based on in-situ bioremediat
ion, which may have a number of strategic advantages including financial be
nefits and environmental sustainability, This paper reviews a range of lite
rature from different scientific disciplines, regarding the physical and bi
ological factors controlling denitrification in fissured aquifers, to asses
s whether in-situ bioremediation of nitrate in Chalk groundwaters is techni
cally feasible. The principal conclusion is that, despite the dual-porosity
operating in this type of aquifer, nitrate removal by engineered, in-situ
bioremediation is potentially a viable and practicable method for treating
groundwater. Process optimization could be achieved by investment in a prog
ramme of fundamental research in a number of key areas. The review highligh
ts the need for a combined experimental and modelling approach, to satisfy
the data and predictive requirements for configuring in-situ bioremediation
systems at the field-scale. It also provides the necessary conceptual basi
s for developing a combined macroscale hydrogeological and biochemical mode
l of denitrification activity in Chalk aquifers.