Investigating the reaction of a novel silica capillary coating compound with proteins/peptides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
M. Galvani et al., Investigating the reaction of a novel silica capillary coating compound with proteins/peptides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, RAP C MASS, 15(3), 2001, pp. 210-216
Quaternized piperazine ((N-methyl-N-omega -iodobutyl-N'-methyl)piperazine;
QPzl) is a novel compound described as an ideal coating material for the si
lica capillaries that are commonly used for capillary zone electrophoresis.
In the course of such analysis, contact between such coatings and biomolec
ules may result in certain modifications of the latter. To gain specific in
formation on such potential modifications, solutions at pH 10.0 containing
both QPzl and standard proteins/peptides were incubated for various periods
and examined by matrix-assisted laser desorption/ionisation mass spectrome
try. The reduction of the S-S bridges, denaturation in 8 M urea, the isoele
ctric point of the protein and the duration of the incubation had a profoun
d influence on the investigated reaction. Analysis in reflectron mode and p
ost source decay identified Cys as the likely site of interaction. The impl
ications of the present measurements for proteome analysis using capillary
and gel electrophoresis are discussed. Copyright (C) 2001 John Wiley & Sons
, Ltd.