Matrix-assisted laser desorption/ionization mass spectrometry using poroussilicon and silica gel as matrix

Porous silicon powder and silica gel particles have been applied as inorgan ic matrices for the analysis of small molecules in matrix-assisted laser de sorption/ionization mass spectrometry (MALDI-TOFMS). In contrast to convent ional MALDI-TOFMS, the signal interference of low-molecular analytes by the matrix has been eliminated. Almost no fragmentations of the analytes were observed. Effects of various factors, such as the particle and pore size, t he suspending solution, and sample preparation procedures, on the intensity of mass spectra have been investigated. The pore structure of the inorgani c matrix and penetration of the analytes into the pores must be optimized f or effective desorption and ionization of the analytes. Matrices (DHB and H CCA) were covalently bound to silica gel for improvement of spectrum intens ity. Copyright (C) 2001 John Wiley & Sons, Ltd.