Increased rectal nitric oxide in coeliac disease after local challenge with gluten

Background: Coeliac disease is an inflammatory disorder characterized by re versible atrophy of small intestinal villi following the ingestion of glute n. Earlier studies indicate that the inflammatory response to gluten may oc cur also very distally in the gastrointestinal tract. The aim of this study was to evaluate whether rectal challenge with gluten would trigger an incr eased local production of the gas nitric oxide (NO), a novel marker of inte stinal inflammation. Methods: Rectal challenge with partially digested glut en was performed in 20 patients with treated coeliac disease and in 13 heal thy controls. Luminal levels of NO were measured in the rectum at 0, 8 and 24 h using a chemiluminescence technique. Results: In patients with coeliac disease mean rectal NO increased from 235 +/- 90 parts per billion (ppb) a t 0 h to 4965 +/- 1653 ppb at 24 h (P < 0.005). In the control group there was no significant increase. One control subject responded with high NO lev els at 24 h and the same individual tested positive for anti-endomysium IgA antibodies. Subsequent duodenal biopsing showed substantial villusatrophy. Conclusions: Rectal challenge with gluten results in increased luminal lev els of NO in a group of patients with treated coeliac disease. Further stud ies are needed to evaluate the role of NO in coeliac disease and the potent ial usefulness of rectal NO measurements in aiding diagnosis of this intest inal disorder.