Mg. Xu et al., Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation, SCANNING, 23(1), 2001, pp. 9-13
Three-dimensional (3-D) imaging of fluorescence resonance energy transfer (
FRET) in human cells under two-photon excitation was demonstrated in this s
tudy. A sample was prepared by expressing a donor and an acceptor in living
cells and using an antibody to secure the proximity of contact between the
donor and the acceptor. The quenching of fluorescence emission of a donor
in the double-labelled cells indicates the presence of FRET that occurred i
n these living cells. Because of the quadratic relation of the excitation p
ower, 3-D localisation of FRET becomes possible.