Specific production of gamma-polypodatetraene or 17-isodammara-20(21),24-diene by squalene-hopene cyclase mutant

Authors
Schmitz, S Full, C Glaser, T Albert, K Poralla, K
Citation
S. Schmitz et al., Specific production of gamma-polypodatetraene or 17-isodammara-20(21),24-diene by squalene-hopene cyclase mutant, TETRAHEDR L, 42(5), 2001, pp. 883-885
Citations number
16
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
42
Issue
5
Year of publication
2001
Pages
883 - 885
Database
ISI
SICI code
0040-4039(20010129)42:5<883:SPOGO1>2.0.ZU;2-Y
Abstract
Amino acids lining the catalytic cavity of squalene-hopene cyclase of Alicy clobacillus acidocaldarius were mutated to investigate their catalytic func tions. Mutagenesis of Leu607 to Lys in the central part of the cavity resul ted in the production of the bicyclic gamma -polypodatetraene (1) as main p roduct, while the mutation of Phe605 to Lys near the deprotonation site of the cavity led mainly to the formation of tetracyclic 17-isodammara-20(21), 24-diene (2). (C) 2001 Elsevier Science Ltd. All rights reserved.