A potential role for interleukin-8 in the metastatic phenotype of breast carcinoma cells

This study shows a strong correlation between the metastatic potentials of breast carcinoma cell lines and their ectopic expression of interleukin-8 ( IL-8). Correlations exist for both constitutive and induced levels of IL-8 released, A correlation was also observed between cell morphology, metastat ic potential, and IL-8 profile. Metastatic lines are fusiform in appearance , whereas, nonmetastatic lines are epithelioid, The metastatic potential of two breast carcinoma lines was examined using an orthotopic model of spont aneous metastasis. Metastatic cells formed rapidly growing, poorly differen tiated primary tumors that metastasized, Nonmetastatic cells formed rapidly growing differentiated primary tumors that did not produce detectable meta stases. Comparison of IL-8 expression by the parental cells and cell cultur es developed from primary and metastatic tumors, demonstrates that IL-8 rel eased by cultured cells from the primary tumor is higher than that of the p arental cells, and IL-8 released by cultured cells derived from the metasta tic lung tumors is greater than that released by cultured cells derived fro m the primary tumor. These data demonstrate a strong correlation between th e metastatic phenotype of a cell and its IL-8 expression, suggesting a role for IL-8 in promoting the metastatic potential of breast tumor cells.