Expression of proinflammatory cytokines in four regions of the brain in Macaque mulatta (rhesus) monkeys infected with Plasmodium coatneyi

Citation
Je. Tongren et al., Expression of proinflammatory cytokines in four regions of the brain in Macaque mulatta (rhesus) monkeys infected with Plasmodium coatneyi, AM J TROP M, 62(4), 2000, pp. 530-534
Citations number
25
Categorie Soggetti
Envirnomentale Medicine & Public Health","Medical Research General Topics
Journal title
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
ISSN journal
00029637 → ACNP
Volume
62
Issue
4
Year of publication
2000
Pages
530 - 534
Database
ISI
SICI code
0002-9637(200004)62:4<530:EOPCIF>2.0.ZU;2-7
Abstract
We have characterized brain cytokine expression profiles in the Plasmodium coatneyi/rhesus (Macaque mulatta) malaria model. Eight rhesus monkeys were included in the study; four were infected with P. coatneyi, and four were u sed as uninfected controls. All inoculated animals became infected. Eleven days after parasite inoculation, the rhesus monkeys were killed and tissue samples from 4 regions of the brain (cortex and white matter of the cerebru m, cerebellum, and midbrain) were collected for quantitation of mRNA expres sion of cytokines, adhesion molecules, and inducible nitric oxide synthetas e (iNOS) by reverse transcriptase-polymerase chain reaction (RT-PCR). The e xpression levels of tumor necrosis actor-alpha (TNF-alpha), gamma interfero n (IFN-gamma), interleukin-l-beta (IL-1 beta), intercellular adhesion molec ule-1 (ICAM-1) and inducible nitric oxide synethetase (iNOS) were highest i n the cerebellum of infected animals, correlating well with pathologic obse rvations of sequestration of parasitized erythrocytes in this region of the brain. Infected animals also had higher TNF-alpha expression levels in the cortex and IL-1 beta expression levels in the cortex, white matter, and mi dbrain. Thus, the expression of pro-inflammatory and T helper-1 (TH-1) cyto kines, adhesion molecules, and iNOS appears to predominate in the cerebellu m of infected rhesus monkeys.