Tr. Larson et Ia. Graham, Application of a new method for the sensitive detection and quantificationof acyl-CoA esters in Arabidopsis thaliana seedlings and mature leaves, BIOCH SOC T, 28, 2000, pp. 575-577
We report a novel, highly sensitive and selective method for the detection
and quantification of acyl-CoA esters from Arabidopsis thaliana. Extracted
acyl-CoA esters were derived to their fluorescent acyl-etheno-CoA esters, s
eparated by ion-paired reversed-phase HPLC, and detected fluorometrically.
We report the composition and concentration of the acyl-CoA pool in mature
A. thaliana leaves, and during storage-lipid breakdown in 2-day-old seedlin
gs. The concentrations measured were in the 1-4 muM range for both tissue t
ypes. To our knowledge, this is the first time that reliable estimates of a
cyl-CoA ester concentrations have been made for plants.