Expression in yeast of an acyl-CoA : diacylglycerol acyltransferase cDNA from Caenorhabditis elegans

Authors
Bouvier-Nave, P Benveniste, P Noiriel, A Schaller, H
Citation
P. Bouvier-nave et al., Expression in yeast of an acyl-CoA : diacylglycerol acyltransferase cDNA from Caenorhabditis elegans, BIOCH SOC T, 28, 2000, pp. 692-695
Citations number
16
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL SOCIETY TRANSACTIONS
ISSN journal
03005127 → ACNP
Volume
28
Year of publication
2000
Part
6
Pages
692 - 695
Database
ISI
SICI code
0300-5127(200012)28:<692:EIYOAA>2.0.ZU;2-4
Abstract
We have identified a cDNA from the nematode worm Caenorhabditis elegans tha t encodes an acyl-CoA:diacylglycerol acyltransferase (DGAT). Its expression in Saccharomyces cerevisiae resulted in an increase both in triacylglycero l content and in microsomal oleyl-CoA: diacylglycerol acyltransferase activ ity. Such effects were similar to those of characterized plant DGAT genes. This is the first DGAT gene isolated from an invertebrate. The phylogenetic relationships between DGATs and animal and yeast acyl-CoA:sterol acyltrans ferases are illustrated.