Because the synthesis of monogalactosyldiacylglycerol (MGDG) is unique to p
lants, identified as an important marker of the plastid envelope, involved
in a key step of plastid biogenesis and is the most abundant lipid on earth
, MGDG synthase activity was extensively analysed at the biochemical and ph
ysiological levels. In the present paper, ave present our current knowledge
on the MGDG synthase's function, structure and topology in envelope membra
nes, and discuss possible roles in plant cell glycerolipid metabolism. The
recent discovery of a multigenic family of MGDG synthases raised the possib
ility that multiple isoenzymes might carry out MGDG synthesis in various ti
ssues and developmental stages.