A family of acyl-CoA oxidase isozymes catalyse the first step in the peroxi
somal fatty acid beta -oxidation spiral. Our group and others have recently
characterized four genes from this family in the model oilseed Arabidopsis
. These genes encode isozymes with different acyl-CoA substrate specificiti
es, which together encompass the full range of fatty acid chain lengths tha
t exist in vivo. Here we review the biochemical properties and physiologica
l roles of the acyl-CoA oxidase isozymes.