Citation
N. Terp et A. Brandt, Lipoxygenases in early Brassica napus germination, BIOCH SOC T, 28, 2000, pp. 832-833
Citations number
6
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL SOCIETY TRANSACTIONS
ISSN journal
03005127
→ ACNP
Volume
28
Year of publication
2000
Part
6
Pages
832 - 833
Database
ISI
SICI code
0300-5127(200012)28:<832:LIEBNG>2.0.ZU;2-4
Abstract
We have identified transcripts of two lipoxygenase (LOXs) from 3-day-germin
ated etiolated cotyledons. One encodes a soluble and the other encodes a pl
astid LOX. They are 2520 bp and 2676 bp in size, respectively. Antibodies r
aised against the C-terminal 500 amino acids of the plastid LOX recognize a
90 kDa LOX in 5-day-old etiolated and green cotyledons. In mature leaves a
LOX of 95 kDa is detected. LOX activity and protein was mainly located in
the cytosol.