Evidence of cross-reactivity between porcine pancreatic and rapeseed (Brassica napus L.) lipases

A cross-reactivity between animal and plant lipases was determined, using i mmunological techniques. It was shown by ELISA and dot-blotting that these antibodies react with lipases in the rapeseed crude extract and in the diff erent cellular fractions obtained by differential centrifugation. Pre-incub ation of the antiserum with the rapeseed crude extract affects the amount o f antibodies binding to the porcine pancreatic lipase. Antibodies were able to precipitate lipase activity from 3-day-old rapeseed crude extract. Thes e epitopes seem to be located in the catalytic site, suggesting that a cons ensus sequence exists in oleaginous Lipases and that it will be universal.