Serum-free recombinant production of adenovirus using a hollow fiber capillary system

A novel rnethod for the production of adenoviral vectors on a scale suffici ent to support most research applications and early phase clinical trials i s presented. This method utilizes serum-free cell culture medium and a holl ow fiber cell culture apparatus. Significantly less time and space are requ ired than in conventional methods, and the resulting adenovirus is collecte d in a much smaller volume, simplifying the purification steps. The protoco l described is a reproducible, convenient, biologically safe, and environme ntally sound method for the production of adenoviral vectors for laboratory nse and has the potential to scale-up the adenovirus production for clinic al use.