One step purification of glucose-6-phosphate dehydrogenase from brain areas by immunoaffinity chromatography

Authors
Capellacci, S Aluigi, G Tabellini, L Ninfali, P
Citation
S. Capellacci et al., One step purification of glucose-6-phosphate dehydrogenase from brain areas by immunoaffinity chromatography, BIOTECH LET, 23(5), 2001, pp. 353-357
Citations number
16
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
5
Year of publication
2001
Pages
353 - 357
Database
ISI
SICI code
0141-5492(200103)23:5<353:OSPOGD>2.0.ZU;2-V
Abstract
Glucose-6-phosphate dehydrogenase was purified from rabbit brain cortex usi ng a single immunoaffinity chromatographic step and was contaminated only b y a 50 kDa protein. The proteins, separated by SDS-PAGE, were sequenced: th e glucose-6-phosphate dehydrogenase was blocked at the N-terminal, the co-e luted protein was similar to alpha -tubulin. Our technique can be applied t o purification and sequencing of the enzyme from brain areas or to measure its turnover rate in cultured cells.