Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system

Authors
Palomares, LA Pedroza, JC Ramirez, OT
Citation
La. Palomares et al., Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system, BIOTECH LET, 23(5), 2001, pp. 359-364
Citations number
20
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
5
Year of publication
2001
Pages
359 - 364
Database
ISI
SICI code
0141-5492(200103)23:5<359:CSAATT>2.0.ZU;2-X
Abstract
The increase of Sf9 cell diameter after infection with a recombinant baculo virus encoding VP8 protein of rotavirus can be used to predict culture prod uctivity. A direct proportional correlation between the increase in cell si ze and VP8 concentration was obtained when manipulating selected medium com ponents. Only yeast extract increased (38%) VP8 concentration, while fetal bovine serum increased (55%) the maximum cell concentration. An inexpensive and simplified culture media can thus be designed without detriment to pro tein yields.