Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticusfor large scale Baeyer-Villiger monooxygenase reactions

Authors
Barclay, SS Woodley, JM Lilly, MD Spargo, PL Pettman, AJ
Citation
Ss. Barclay et al., Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticusfor large scale Baeyer-Villiger monooxygenase reactions, BIOTECH LET, 23(5), 2001, pp. 385-388
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
5
Year of publication
2001
Pages
385 - 388
Database
ISI
SICI code
0141-5492(200103)23:5<385:POCMFA>2.0.ZU;2-N
Abstract
Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus g rown on a medium containing both glutamate (30 g l(-1)) and cyclohexanol (1 g l(-1)). Productivity was increased to 650 U l(-1), an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.